Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated

Goat anti-Human IgG + IgA + IgM, F(ab)'2 fragment, HRP conjugated

APA-GA-068

Goat

Goat anti-human IgG + IgA + IgM, HRP conjugated is a F(ab)'2 fragment, of a secondary antibody which binds to human IgG+IgA+IgM in immunological assays.  Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

Lyophilized

Polyclonal

Immunogen affinity purified IgG, F(ab)'2 fragment.

0.5 mg

Human IgG + IgA + IgM

For reconstitution add 0.55 ml of sterile water

1 : 500-1 : 5000 (IHC), 1 : 10 000-1 : 50 000 (WB)

Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.