Product Name
ALD | Fructose-1,6 bisphosphate aldolase
Immunogen
overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930
Background
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Reactivity
A. thaliana, B. napus, C. arietinum, E. tef, G. gracilis, O. sativa, P. falciparum, T. salsuginea
Confirmed reactivity
Arabidopsis thaliana, Brassica napus, Cicer arietinum, Eragrostis tef, Gracilaria gracilis (red algae), Oryza sativa, Plasmodium chabaudi, Plasmodium falciparum, Thellungiella salsuginea
Predicted reactivity
Glycne max, Nicotiana attenuata, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Physcomitrium patens, Pisum sativum, Populus jackii, Spinacia oleracea, Vitis vinifera, Zea mays
Not reactive in
Synechocystis sp.
Reconstitution
For reconstitution add 100 µl of sterile water
Expected/apparent MW
38 | 38 kDa
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.