H+ATPase | Plasma membrane H+ATPase (chicken antibody)

H+ATPase | Plasma membrane H+ATPase (chicken antibody)

Product Name
H+ATPase | Plasma membrane H+ATPase (chicken antibody)
Cat#
APA-GA-204
Host
Chicken
Immunogen
KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3).
Background
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Format
Lyophilized
Clonality
Polyclonal
Purity
Immunogen affinity purified IgY in PBS pH 7.4.
Quantity
50 µg
Reactivity
Global antibody for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane
Confirmed reactivity
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Predicted reactivity
Angomonas deanei, Avena sativa, Brassica napus, Citrus limon, Coffea canephora, Cucumis sativus, Cucurbita moschata, Dunaliella spp, Eichhornia crassipes, Emiliana huxleyi, Glycine max (weak), Hordeum vulgare, Lactobacillus johnsonii, Laishamania braziliensis, Nicotiana tabacum, Oryza sativa, Solanum lycopersicon, Solanum tuberosum, Medicago truncatula, Mesembruanthemum crystallinum), Nannochloropsis gaditana CCMP526, Nepenthes alata, Nicotiana tabaccum, Nitrospira bacterium, Oryza sativa, Ostreococcus spp., Phaseolus acutifolius, Physocomitrella patens, Picea abies, Pinus thunbergii, Populus tremula, Pteris vittata, Ricinus communis, Saccharomyces cerevisiae, Solanum lycopersicum, Strigomonas culicis, Toxoplasma gondii, Triticum urartu, Trypanosoma cruzi, Zosteria marina, Vicia faba, Vigna angularis
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
95 kDa (Arabidopsis thaliana)
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000-1 : 5000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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