Product Name
AOX1 | Algal Alternative oxidase 1
Immunogen
whole presumed mature AOX1 protein from from Chlamydomonas reinhardtii UniProt: O65000 fused to GST
Background
The mitochondrial AOX (alternative oxidase) of the unicellular green alga Chlamydomonas reinhardtii is encoded by two different genes, the Aox1 and Aox2. The alternative respiratory pathway is comprised of a single homodimeric protein – AOX – and functions as a mechanism to decrease the formation of reactive oxygen species (ROS) produced during respiratory electron transport. Alternative oxidase expression is influenced by different stress stimuli.
Reactivity
Chlamydomonas reinhardtii
Confirmed reactivity
Chlamydomonas reinhardtii
Predicted reactivity
Aspergilus niger, Gonium pectorale, Monoraphidium neglectum, Nannochloropsis gaditana, Ostreococcus lucimarinus, Tetrabaena socialis, Volvox carteri f. nagariensis
Not reactive in
Cyanidioschyzon merolae
Reconstitution
For reconstitution add 50 µl of sterile water
Expected/apparent MW
36 | 36 kDa (Chlamydomonas reainhardii)
Tested applications
Western blot (WB)
Recommended dilution
1 : 10 000 (WB)
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.