3' Primer
pBABE 3' primer: ACCCTAACTGACACACATTCC
5' Primer
pLXSN 5' primer: CCCTTGAACCTCCTCGTTCGACC
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
DH5α, HB101 etc.
Host cells (lines)
Mammalian cells
Selectable Marker
Puromycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Viral/Non-Viral
Retrovirus
Plasmid Type
Retrovirus vectors, RNAi vectors