3' Primer
M13 fwd primer: TGTAAAACGACGGCCAGT
5' Primer
CMV fwd primer: CGCAAATGGGCGGTAGGCGTG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
ccdB positive screening DB3.1 competent cells, negative screening with OmniMAX2-T1 competent cells
Selectable Marker
Neomycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Plasmid Type
Mammalian expression vectors