5' Primer
T7 Fwd:Â TAATACGACTCACTATAGGG
Cloning Method
MCS, Restriction endonucleases
Cloned Strain(s)
ccdB positive screening DB3.1 competent cells, negative screening with OmniMAX2-T1 competent cells
Host cells (lines)
Conventional cell lines, 293, CV-1, CHO, etc.
Stable
Transient or stable expression
Selectable Marker
Neomycin
Constitutive/Inducible
Constitutive
Bacterial Resistance(s)
Ampicillin
Tag
V5 epitope (C-terminal), 6X His tag (C-terminal)
Plasmid Type
Vectors, Mammalian cell expression vectors