Single Nucleotide Polymorphism Technology Service

Single Nucleotide Polymorphism (SNP) is a DNA sequence polymorphism caused by the variation of a single nucleotide at the genomic level. SNPs include single base conversions, reversals, insertions, and deletions. SNPs are the third generation of DNA-level genetic polymorphism markers after RFLP, VNTR, and STR.

Figure 1. Model for the Role of SNPs in pMdhpRNA277 in Regulating A. alternata Leaf Spot Resistance in Apple. (Qiu lZ, et al. 2018)

• Technical Characteristics
• High density. SNP densities are higher than microsatellite markers, providing a range of markers within or near any of the genes to be studied.
• Genetic stability. SNPs have higher genetic stability than repetitive sequence polymorphic markers such as microsatellites.
• Easy automation of analysis. SNPs do not require measurements of fragment length, which makes SNP-based detection and analysis methods easy to automate.
• What We Offer

Lifeasible has advanced instrumentation and years of experience in plant molecular biology and can provide you with rapid and comprehensive SNP detection and mutation analysis services using Direct sequencing method, KASP typing method, LDR typing method, Multiplex SNaPshot SNP typing method, Taqman probe method, etc.

Typing Method	Technical Characteristics
Direct sequencing method	The highest accuracy, "gold standard", large workload, suitable for a small number of samples, many points, and centralized typing detection.
KASP typing method	It is suitable for typing detection with a large sample size and few loci.
ligase detection reaction (LDR) typing method	It is suitable for any polymorphic site, and there is no need to introduce restriction sites artificially. Accurate typing (> 95%), high detection rate, short cycle, and high performance-to-price ratio.
Multiple SNaPshot SNP typing method	The typing is accurate (> 98%), and multiple sites can be detected at the same time, which is not limited by the polymorphic characteristics of SNP loci and the number of samples.
Taqman probe method	The use of an MGB probe can greatly improve the signal-to-noise ratio and is suitable for detection with a small number of sites and high sample flux. The operation is simple, only a one-step PCR reaction is needed, no purification and pretreatment are needed, and the detection is directly on the computer with high accuracy.

• Service Flow



• Why Choose Lifeasible
• Efficient and fast. Thousands of SNP loci can be genotyped per reaction and thousands of sample markers can be mixed simultaneously.
• High accuracy. Due to the high specificity of ligase recognition, non-specific linkage products can be excluded by sequencing data analysis, which greatly improves the accuracy of the data.
• Personalized service. We have accumulated rich experience in SNP genotyping assays and can design personalized assays according to customer needs.
• Short cycle time. We have a variety of assay platforms to achieve rapid and stable SNP genotyping.
• Cost-effective. As always, we provide the most comprehensive and professional services to our customers at the best price.
• How to Place an Order

Lifeasible offers complete, professional SNP technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact Lifeasible.