Inter-simple Sequence Repeat (ISSR) is a new molecular marker technique developed based on SSR. Its basic principle is to add 2-4 random nucleotides to the 3' or 5' terminal of SSR sequence, amplify a sequence with the reverse arrangement of SSR on both sides by PCR, and then use electrophoresis technology to analyze its polymorphism according to the presence or absence of bands and their relative position.
Figure 1. ISSR profiles of 33 genotypes of citrus using a primer (AG)8YT (primer 10). (Shahsavar AR, et al. 2007)
ISSR markers are based on the widespread presence of SSRs in plants, and primers are designed using the SSRs themselves, which are commonly found in plant genomes, without the need for pre-cloning and sequencing. The primers used for ISSR-PCR amplification are usually 16 to 18 base sequences, consisting of tandem repeats of 1 to 4 bases and several non-repetitive anchor bases, thus ensuring that the primers bind to the 5' or 3' terminal of the SSRs in genomic DNA, resulting in repeats located in reverse alignment and not too widely spaced between of genomic segments for PCR amplification.
Since ISSR molecular markers combine the advantages of other molecular markers and break the limitations of several other molecular marker techniques to a certain extent. Compared with other molecular markers, ISSR molecular markers have many advantages such as simple operation, high safety, low cost, low amount of template DNA, no need for sequencing and primer design, rich polymorphism, easy operation, high stability, faster and more convenient, and wide application. Therefore, it is increasingly used in various fields of plant research.
Lifeasible provides ISSR technical services for the study of plant genetic diversity. Provide customers around the world with the following optional research solutions (including but not limited to), which can be freely combined according to the content of the project. Besides, our experts are always ready to provide you with customized personalized services.
Lifeasible, as a world-leading phytotechnology company, has rich experience in ISSR assay analysis and a mature and advanced platform. We provide a full set of ISSR assay analysis services from experimental design to data analysis. After the end of the experiment, our customers can get the original datasheet, original picture, primer sequence, professional analysis results, and complete experimental report as soon as possible, and there will be a special person to do after-sales service in the follow-up. If you have any questions about the experimental results, you can contact our staff at any time.
Lifeasible offers complete, professional ISSR technology service, as well as customized experimental protocols based on your project requirements and plant sample characteristics. For more information, please contact Lifeasible.
Reference:
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
