Enzymes in plants affect the growth, division, and differentiation of plant cells and can determine rooting, germination, flowering, fruiting, dormancy, and abscission. Plant enzymes play an essential role in regulating and controlling the growth and development of plants. Plant enzyme activity refers to the concentration and activity degree of the enzyme in the plant, and its regulation and control is an important feature that distinguishes it from general catalysts. Plant enzyme activity assay is an indispensable part of plant growth and metabolism. Since enzymes have a high degree of specific catalytic activity, their activity can be determined by measuring the change in the concentration of the corresponding substrate or product or the change in the concentration of a reaction product or reactant. Lifeasible provides professional plant enzyme activity testing services for global customers, including glycolysis, glyoxylate cycle, oxidation and antioxidation, coenzymes, etc. The efficiency is higher for multiple enzymes together.
As an expert in plant biochemical analysis, Lifeasible provides customers with comprehensive solutions for plant enzyme activity testing. We have established a highly efficient and stable enzyme activity assay system to detect the content of various types of enzymes by ELISA method or to detect enzyme activity by biochemical method.
Below are the specifics of our plant enzyme activity testing:
Test Items | Test Methods |
Superoxide Dismutase (SOD) Catalase (CAT) Peroxidase (POD) Malondialdehyde (MDA) Polyphenol oxidase (PPO) Phenylalanine ammonia-lyase (PAL) Hydrogen peroxide (H2O2) Superoxide anion (O2-) Ascorbate peroxidase (APX) Ascorbic acid oxidase (AAO) Reduced glutathione (GSH) Oxidized glutathione (GSSG) Glutathione S-transferase (GST) Glutathione reductase (GR) Nitrate reductase (NR) Glutaminase (GLS) Nitrite reductase (NiR) Reduced Ascorbic Acid (AsA) Oxidized/Dehydroascorbic Acid (DHA) Total Ascorbic Acid (TAA) α-Amylase β-Amylase Total Amylase Glutamine aminotransferase/alanine aminotransferase (GPT/ALT) Glutamyl Transaminase/Aspartate Aminotransferase (GPT/ALT) Hydroxyl Radical Scavenging Capacity ABTS Scavenging Capacity DPPH scavenging capacity Superoxide anion radical scavenging capacity Glutathione peroxidase (GSH-Px/GPX) Total antioxidant capacity (T-AOC) Alkaline Protease Acidic Protease Neutral protease Leucine Aminopeptidase Tannase Dehydrogenase (PDHA) Non-protein sulfhydryl groups (NPTs) Protein Carbonyls (PCO) Total Thiols (TTs) Nitric Oxide(NO) Anti-lipid peroxidation capacity Polyamine Oxidase (PAO) NADPH oxidase (NAO) Methylglyoxal (MG) Glyoxalase I (Gly I) Glyoxalase II (Gly II) Ferric iron reductase (FCR) β-Glucosidase (β-GUS)/β-Glucuronidase (β-G) β-1,3-1,4-Glucanase Pullulanase Alginate synthase (TS) Alginose-6-phosphate synthase (TPS) Alginate-6-phosphate esterase (TPP) Sorbitol oxidase (SOX) Glucosamine 6-phosphate synthase (GlmS)/Fructose 6-phosphate glutamine aminotransferase (GFAT) Sorbitol 6-phosphate dehydrogenase (S6PDH)/aldose 6-phosphate reductase (A6PR) Fructan hydrolase Pyruvate phosphate dikinase (PPDK) Pyruvate phosphate dikinase (PPDK) Adenosine Deaminase (ADA) Asparaginase (ASNase/asparaginase) Asparagine synthetase (ASNS) Aryl Amidase Starch phosphorylase Vitamin B1 (VB1) Vitamin B6 Vitamin E NADH-Glutamate synthase (NADH-GOGAT) Fd-glutamate synthase (Fd-GOGAT) NADH-Glutamate dehydrogenase (NADH-GDH) NADPH-Glutamate dehydrogenase (NADPH-GDH) Glutamate synthase (GOGAT) Glutamate dehydrogenase (GDH) Endonuclease Exonuclease Chitinase NAD+-sorbitol dehydrogenase (NAD+-SDH) NADP+-Sorbitol dehydrogenase (NADP+-SDH) Sorbitol dehydrogenase Hemicellulase/Xylanase Acid xylanase Neutral xylanase Alkaline xylanase Pyruvate decarboxylase (PDC) ATP α-Ketoglutarate dehydrogenase (α-KGDH) β-Glucan Acyltransferase (AAT) 6-Phosphogluconate dehydrogenase (6PGDH) NADP-Malate dehydrogenase (NADP-MDH) NAD-Malate dehydrogenase (NAD-MDH) Glucose 6-phosphate dehydrogenase (G6PDH) Citrate synthase(CS) Plant sucrase Glutamic acid decarboxylase (GAD) Ethanol dehydrogenase (ADH) Manganese peroxidase (Mnp) Lignin peroxidase (Lip) Lipase(LPS) Trypsin L-lactic acid (L-LA) Hydroxyl radical (OH-) D-Lactic Acid (D-LA) Phosphofructokinase (PFK) Pyruvate kinase (PK) activity Hexokinase (HK) Reactive Oxygen Species (ROS) Hydrogen Sulfide(H2S) Monoamine oxidase (MAO) Diamine Oxidase (DAO) Indoleacetic acid oxidase (IAAO) Tyrosine aminotransferase (TAL) Xanthine oxidase (XOD) Lactate dehydrogenase (LDH) Phenol oxidase (PO) Phenol oxidase (PO) Thioredoxin oxidoreductase (TrxR) Thioredoxin peroxidase Dehydroascorbate reductase (DHAR) Monodehydroascorbate reductase (MDHAR) γ-Glutamylcysteine synthetase (γ-GCS)/γ-Glutamylcysteine ligase (GCL) Glutamine synthetase (GS) Urease (UE) γ-Glutamyl transpeptidase (γ-GT) Sucrose synthase SS-II Sucrose synthase SS-I Sucrose phosphorylase (SPase) Sucrose phosphate synthase (SPS) Neutral Invertase (NI)/Cytoplasmic Invertase Soluble acidic invertase (S-AI)/vesicular invertase Cell wall insoluble acidic invertase (B-AI) UDPG pyrophosphorylase/uridine diphosphate glucose pyrophosphorylase ADPG pyrophosphorylase/adenosine diphosphate glucose pyrophosphorylase Soluble starch synthase (SSS) Conjugated starch synthase (GBSS) Starch branching enzyme (SBE) α-Glucosidase (α - GC) β-Glucosidase (β-GC)/Fibre disaccharidase α-Galactosidase (α-GAL) β-Galactosidase (β-GAL) Glucose dehydrogenase (GCDH) N-acetyl-β-D-aminoglucosidase (NAG) Endo-β-1,4-glucanase/cellulase Exo-beta-1,4-glucanase/cellobiose hydrolase (CBH)/cellobiose glycosidase Filter paper enzyme(FPA) Laccase (Lac) β-1,3 Glucanase(β-1,3-GA) Glucose-6-Phosphatase(G6Pase) Trehalase (Alginase) Glycogen synthase (GCS) Glycogen phosphorylase a (GPa) Glycogen phosphorylase b (GPb) Glycosylase kit Ribulose diphosphate carboxylase(Rubisco) Plant fructose-1,6-bisphosphate aldolase(FBA) Plant fructose-1,6-bisphosphate (ester) enzyme (FBP) Phosphoenolpyruvate carboxylase (PEPC) Fructose-6-phosphate-1-phosphotransferase (PFP) Glyceraldehyde 3-phosphate dehydrogenase (NADPH-GAPDH) Glycolate oxidase (GO) Chloroplast 3-phosphoglycerate kinase (PGK) Polygalacturonase (PG)/Pectinase Pectin Lysase(PL) β-Xylosidase kit α-L-Arabinofuranosidase Cinnamic acid 4-hydroxylase(C4H) Cinnamyl alcohol dehydrogenase(CAD) Starch debranching enzyme (DBE) Cell wall-bound acid converting enzyme Glucose oxidase (GOD), etc. |
Ultraviolet absorption method Kits Guaiacol assay Nitrogen blue tetrazolium assay Determination by spectrophotometer method |
Lifeasible provides comprehensive content of plant enzyme activity analysis and can design customized solutions according to your specific requirements. We hope that our products and technology can satisfy our customers. We look forward to your inquiry. For more information, please contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024