With more than 130 alkaloids isolated from the periwinkle plant, many of which have antitumor activity, the most valuable of which is Vincristine, the sulfate salt of which has been used in the clinic. Lifeasible, a leader in plant research and development, has made clear the importance of comprehensive analytical services for Vincristine and its related compounds. Our state-of-the-art laboratories and experienced team of scientists provide cutting-edge analytical solutions for the comprehensive characterization and profiling of vincristine.
Lifeasible's analysis of periwinkle alkaloids requires pre-treatment services for periwinkle alkaloids, including extraction, analysis, and purification.
Crude powder of periwinkle was stirred with water, left for a moment, cold immersed with benzene, extracted with tartaric acid, and filtered. The filtrate was alkalized with ammonia extracted with chloroform; the total alkaloid was added with methanol and left overnight, precipitated, and filtered, and crystallized as isovincristine. The filtrate was decompressed to recover methanol and purified. Total alkaloids plus anhydrous ethanol and 5% sulfuric acid anhydrous ethanol solution, left for two days to precipitate crystals filtered crystals (vincristine, vincristine mixture), dissolved in water, ammonia alkalization, chloroform extraction, chloroform solution with anhydrous sodium sulfate dehydration, concentration, drying, chromatography by alumina columns, with a mixture of benzene, chloroform, elution, and collection of segments.
The first section was evaporated and dried plus 5% sulfuric acid anhydrous ethanol as periwinkle sulfate, the middle section was subperiwinkle, and the latter section was evaporated and dried plus 5% sulfuric acid anhydrous ethanol as periwinkle neopine sulfate.
The volumetric method is the most popular method, but also can be used in gravimetric, colorimetric, visible, and ultraviolet spectrophotometry, chromatography, etc. In volumetric method, acid-base titration is the most popular method. Among the volumetric methods, the acid-base titration method is simpler and commonly used. The periwinkle alkali obtained by extraction and refining is dissolved in excess of the standard acid solution, and the content of periwinkle alkali is calculated from the amount of acid consumed by titrating back with the standard alkali solution.
Vinpocetine components are analyzed by Thin Layer-UV Spectrophotometry, Thin Layer Scanning, HPLC, Non-Aqueous Capillary Electrophoresis, HPLC-ESI/MS coupling, Electrochemical Analysis, etc. Lifeasible offers a wide range of characterization services to meet the specific needs of our customers. Our advanced analytical techniques combined with high-resolution instrumentation allow for precisely identifying and quantifying vinblastine and its impurities. By employing periwinkle analysis, the complex molecular composition of periwinkles can be elucidated to ensure their quality and efficacy.
Lifeasible is proud of its commitment to excellence in vinblastine analysis services. Our company's expertise and state-of-the-art facilities enable us to deliver high-quality results with the utmost precision and efficiency. Combining our advanced analytical techniques, extensive experience, and dedication to customer satisfaction, we strive to be at the forefront of vinblastine research and development. If you are interested in our services or have questions, please feel free to contact us or make an online inquiry.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
