Detection of Plant Nematodes by PCR

Based on the idea of polymerase chain reaction, PCR technology was proposed. Since then, this technique has become one of the most commonly used and important molecular biology techniques. The double-stranded DNA undergoes denaturation at high temperatures to become two single strands, and at low temperatures, the two single strands become a double strand. Therefore, the denaturation and renaturation of DNA can be controlled by the change in temperature so that the in vitro replication of a specific gene can be realized, which is called PCR technology.

Lifeasible provides the detection of plant nematodes by polymerase chain reaction (PCR) to assist our customers in molecular diagnosis and basic research on the pathogenicity of plant nematodes. We develop an advanced platform equipped with advanced instruments and professional staff to perform proteomic analysis with a high standard. We deliver satisfactory and reliable results and reports on time to our customers worldwide.

Detection of Plant Nematodes by Conventional PCR

• Molecular diagnostic methods based on sequence differences have become a common approach in plant pathology.
• Lifeasible provides detection of plant nematodes with conventional PCR, including design for specific primers, PCR operations, and results reporting. PCR technology can be used to distinguish morphologically similar nematodes by amplifying specific regions, and using different primers for detection.
• In addition, we help our customers to realize the batch detection of plant nematodes through PCR technology.

Fig.1 The process of detecting plant nematodes by PCR.

Detection of Plant Nematodes by Nested PCR

• Toto improve the specificity of PCR amplification, the technique of conventional PCR is improved, so nested PCR appears in practice. It is done by adding and amplifying a set of primers to improve the specificity of PCR.
• We provide detection of plant nematodes with nested PCR, which can detect an individual nematode about the size of an egg. This method is proved to be a highly specific and sensitive detection method.

Detection of Plant Nematodes by Real-Time PCR

• In real-time PCR technology, fluorescent dyes or probes with fluorescent groups are added to the reaction system. The real-time PCR progress can be monitored by the accumulation of fluorescent signals after each amplification cycle.
• We provide detection of plant nematodes with real-time PCR technology, which has become a more commonly used method for identifying nematode species due to its advantages of sensitivity and accuracy.
• Based on real-time PCR technology, our services include nematode nucleic acid extraction, specific fragment detection, result in interpretation and report output.

Lifeasible offers the detection of plant nematodes by PCR to meet your research demands. With years of experience in the biological industry, our professional platforms can help our clients solve types of difficulties. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.