Detection of Plant Nematodes by Mass Spectral Analyses

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Pathogenic nematode protein profiles can help researchers to understand how pathogenic nematodes interact with their hosts. Matrix-assisted laser desorption/ionization (MALDI) is an ionization technique which uses a laser energy-absorbing matrix to produce gaseous ions from large molecules in the solid state. Molecules are ionized by the loss/gain of protons in the hot plume of ablated gases and accelerated into a mass spectrometer for detection. Time of flight mass spectrometer (ToF-MS) measures the time taken by these ions to reach the detector, which is measured by the mass/charge value, with smaller and/or more charged ions moving faster.

Lifeasible is a credible provider of high-quality detection of plant nematodes by mass spectral analyses. Our platform is equipped with state-of-the-art facilities and highly experienced staff to support projects in all areas. We guarantee to deliver results on time to our customers.

Detection of Plant Nematodes by Mass Spectral Analyses

The basis of taxonomic identification using MALDI-ToF-MS is the ability to detect protein/peptide ions or protein profiles that are diagnostic to the taxa being considered.
Lifeasible provides detection of plant nematodes with MALDI-ToF-MS, a rapid and simple approach based on the analysis of protein profiles of plant nematodes.
In addition, we provide MALDI-ToF-MS coupled with 2-DGE to provide a powerful tool in nematode taxonomy. The methods allow for inferences to be made regarding evolutionary relationships among taxa as well as for the development of species-specific markers.

Fig.1 Protein profiles of plant nematodes with MALDI-ToF-MS. (Perera MR, et al., 2005)

Workflows of Mass Spectral Analyses

Steps	Operation Methods
Protein Extraction and Sample Preparation	Proteins are extracted by grinding nematodes in 15 mL of the solvents or solvent combinations. After 40 min to 1 hour of extraction, the extract is centrifuged at 5200 g for 1 min in a microcentrifuge to pellet the debris. The supernatant (10-12 mL) is removed, placed in a 0.5 mL microcentrifuge tube, and vacuum-dried in a concentrator. The dried extract is resuspended in 10 mL of the matrix solutions.
Optimization of Instrument Settings for MALDI-ToF MS	The sample/matrix solution is mixed by vertexing, and then 1.5 mL of the sample is spotted onto a sample plate and air-dried. Air-dried samples are analyzed in linear mode. Mass spectra are collected manually or automatically using a range of acceleration voltages, grid wire voltages, plate voltages, extraction delay times, and acquisition masses. The laser intensity is adjusted until acceptable spectra are obtained.

Lifeasible provides fast turnaround, high-quality detection services at competitive prices for customers worldwide. Our advanced technical platforms can help our clients complete the research process with quality and quantity. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.

Reference

Perera MR, et al. (2005). "A novel approach to identify plant parasitic nematodes using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry." Rapid Commun Mass Spectrom. 19 (11), 1454-60.

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