Genomic Analysis of Plant Nematodes

Plant nematodes are one of the main pathogens of crops, which cause significant crop yield reduction and serious economic losses every year. With the wide application of high-throughput sequencing technology, the genomes of many essential plant nematodes have been decoded and reported. Based on this, the origin and evolution of plant nematodes have been further revealed by comparative genomics, and a series of significant advances have been made in functional genomics.

Lifeasible is a credible provider of comprehensive, high-quality services for plant science. We provide an advanced and professional platform for the genomic analysis of plant nematodes according to customer needs. We hope to partner with you to explore new frontiers in plant science and accelerate your discovery.

Genome Sequencing and Characteristics of Plant Nematodes

• The genome of Caenorhabditis elegans is the first nematode genome to be sequenced, and its results provide an essential reference for subsequent studies on the genomics of plant nematodes. With the application of high-throughput sequencing technology, more and more plant nematode genomes have been reported.

Fig.1 Example of three allelic regions in the M. incognita assembly. (Abad P and McCarter JP, 2011)

• Lifeasible provides genome sequencing for plant nematodes, including flow cytometry, Cot analysis, second-generation sequencing technology, and third-generation sequencing technology. We also help our clients analyze the sequencing results, such as assembly size, chromosome number, and No. of Scaffolds, Scaffolds N50, CEGMA complete, BUSCO complete, genes, and GC%.

Analysis of Tandem Repeat Sequences

• Tandem duplication of genes is a means to avoid or overcome host resistance during the evolution of the interaction between pathogen and host. The increase or decrease of repeat sequences may lead to a change in gene function. Therefore, tandem repeats play a very important role in the genome evolution of plant nematodes.
• We provide multiple analyses of tandem repeat sequences in plant nematodes, such as the MAP-1 effector protein, CLAVATA / ESR (CLE), the GLAND 18 effector protein, and so on. We also help analyze the proportion of repetitive sequences, the proportion of low complexity regions, repetitive units, and their specific functions.

Analysis of Horizontal Gene Transfer

• Horizontal gene transfer (HGT) plays an important role in the evolution of plant nematodes, and many degrading enzymes of plant cell walls are originally obtained from bacteria through HGT.
• We provide studies of functional proteins obtained by horizontal gene transfer in nematodes, such as β-1, 4-endoglucanase, cellulase, glycoside hydrolase, glycosyltransferase, glycosyl binding protein, and expansion, which play key roles in the process of root migration, feeding, and plant cell wall modification during development.

Analysis of Gene Family Expansion

We provide analysis of the expansion of gene families, mainly including large gene families in plant nematodes, such as G-protein-coupled receptors (GPCRs), G-protein alpha subunits (GPA), SPRY domain protein families, CAZyme, etc.

With years of experience in biological services, our advanced platforms can help our clients solve various difficulties in the research of plant nematodes. Lifeasible provides our clients with direct access to our experts and responses to their questions. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.