Analysis of Calreticulin in Plant Nematodes

Various food crops, vegetables, fruits, and ornamental plants in tropical, subtropical, and temperate regions are infected and damaged by plant nematodes. Calreticulin (CRT) is a highly conserved and functional protein family widely found in higher organisms. Belonging to the endoplasmic reticulum retention protein family, CRT can be isolated in vertebrates, invertebrates, and higher plants. It usually contains three major internal domains, consisting of a globular N domain and two Ca²⁺ binding domains.

Lifeasible, a leading global company, is committed to helping our customers achieve effective and successful research. We provide an analysis of calreticulin in plant nematodes, including synthesis, secretion, and various functions, achieving the purpose of controlling nematode diseases. We always deliver reliable results and reports on time to our customers worldwide.

Synthesis and Secretion Analysis of Calreticulin in Plant Nematodes

• Calreticulin was first discovered in Meloidogyne incognita, and then calreticulin genes of other plant nematodes were cloned and reported one after another.
• The life cycle of plant nematodes can be divided into different stages. Understanding the expression levels of calreticulin in plant nematodes at different ages is helpful in understanding its functions. In addition, calreticulin is synthesized at different sites in plant nematodes, which may be related to the different functions that calreticulin plays in different plant nematodes.

Fig.1 Structure of calreticulin.

• Lifeasible provides synthesis and secretion analysis of calreticulin in plant nematodes from three aspects, including the site of calreticulin synthesis in plant nematodes, the developmental expression of calreticulin at different instar stages in plant nematodes, and the localization of calreticulin in plant cells.
• In addition, we help our clients with various calreticulin in plant nematodes, including Mi-CRT, CRT 1, and others.

Functional Analysis of Calreticulin in Plant Nematodes

• Silencing of calreticulin related genes significantly reduced nematode infestation. These results suggest that plant nematodes require calreticulin to promote their parasitism and reproduction under unsuitable environmental pressures.
• Calreticulin belongs to the family of endoplasmic reticulum retention proteins, and the endoplasmic reticulum is an important site of Ca²⁺ storage. However, Ca²⁺ usually acts as a second messenger and plays a crucial role in numerous signaling pathways in animal and plant cells.
• We provide functional analysis of calreticulin in plant nematodes for our customers, including promoting nematode parasitizing, inhibiting plant immune response, and interfering with calcium signal transduction.

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