Genetic Engineering Modification for Nematode Resistant Varieties

Plant nematodes always cause serious damage and a reduction in crop yields. Most of the nematodes are obligate biotrophic parasites of plants, and their infection results in above-ground symptoms in plants like leaf necrosis, chlorosis, plant wilting, and stunted growth. It is essential to devise various management strategies against plant nematodes in economically important crop plants. Genetic engineering has proven a promising tool for developing biotic and abiotic stress tolerance in crop plants. Additionally, the genetic engineering leading to transgenic plants harboring nematode resistance genes has demonstrated its significance in plant nematology.

Lifeasible provides a range of services to our customers worldwide, including transgenic strategies based on natural resistance genes, proteinase inhibitor coding genes, anti-nematode coding genes, and RNA interference for targeting plant nematodes. With extensive experience and expertise in plant science, we are committed to providing you with timely and high-quality deliverables.

Transgenic Strategy Based on Natural Resistance Genes

• Using natural host resistance to control nematode parasitism on crops has been the preferred strategy as an environmentally benign and more cost-effective alternative to chemical control. 'Resistance' as applied to nematode control is normally interpreted as meaning the prevention or limitation of nematode multiplication as a result of the expression of specific host genes.
• Lifeasible provides a transgenic strategy based on natural resistance genes, such as Hs1pro-1, Gpa, Gro, Hero, Rhg, Mi-1, and others. In addition, we also help analyze the elements of the resistance response mediated by natural resistance genes.

Fig.1 Genetic engineering modification.

Transgenic Strategy Based on Proteinase Inhibitor Coding Genes

• Proteinase inhibitors (PIs) are molecules, mostly protein in nature, which inhibit the function of proteinases/proteases released by pathogens. After the attack of herbivores and wounding, a variety of proteinase inhibitors are produced in the plants.
• In the case of plant nematodes, these PIs become active against all four classes of proteinases from nematodes, including serine, cysteine, metalloproteinases, and aspartic. We offer a transgenic strategy based on proteinase inhibitor coding genes, including cowpea trypsin inhibitor (CpTI), serine PI (SpTI), cystatins, and others.

Transgenic Strategy Based on Other Anti-Nematode Coding Genes

Although proteinase inhibitors have shown the most promise in conferring nematode resistance in transgenic plants, several other classes of proteins with putative anti-nematode activity have also been investigated. We also provide a transgenic strategy based on other anti-nematode coding genes, including lectins, antibodies, Bt crystal proteins, and peptides that disrupt chemoreception in nematodes.

RNAi for Breeding Nematode Resistant Varieties

RNA interference (RNAi) has emerged as a very useful tool for gene-silencing aimed at the functional analysis of different genes by suppressing their expression in various organisms, including plant nematodes. We also provide RNAi services targeting plant nematodes via double-stranded RNA (dsRNA) or short interfering RNAs (siRNAs).

Lifeasible has been dedicated to planting science for many years. We'll finish your studies on time and within budget. We guarantee the confidentiality and sensitivity of our customer's data. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.