Transgenic Strategy Based on Natural Resistance Genes

Using natural host resistance to control nematode parasitism on crops has been the preferred strategy as an environmentally benign and more cost-effective alternative to chemical control. Natural resistance genes could exist in both a polygenic manner and single dominant nature. The resistance conferred by host plant single dominant resistance genes, the R genes from plants, interacts specifically with corresponding avirulence (Avr) genes in the nematode, resulting in a so-called 'gene-for-gene' interaction. This type of interaction initiates a cascade of defense responses in plants.

Lifeasible provides a range of services to our customers worldwide, covering transgenic strategies based on natural resistance genes. With extensive experience and expertise in plant science, we are committed to providing you with timely and high-quality deliverables.

Natural Resistance Genes from Other Plant Species

• It is reported that transgenic strategies have been used to enhance nematode resistance in plants. Several natural host resistance genes have been cloned from some plant species and could be transferred to other species. Transgenic expression of resistance proteins also induces the expression of PR (pathogenesis related) proteins to establish nematode resistance in plants.
• Lifeasible provides transgenic strategies based on natural resistance genes from other plant species, which can attack plant nematode infection. Our transgenic strategies include Mi gene and Hero A from tomato, Hs1pro-1 from sugar beet, Gpa-2 from potato, and Cre loci from grass.
• We also provide transgenic services, such as RNA isolation and cDNA synthesis, production of transgenic lines containing natural resistance genes, plant nematode tests in the dishes, plant nematode staining with a fluorescent dye, and others.

Fig.1 Transgenic strategy for resistance to plant nematodes.

Natural Resistance Genes from Own Plant Species

• In some examples, the constitutive expression of genes has increased resistance in plants against nematodes.
• We provide transgenic strategies based on natural resistance genes from our own plant species, such as Gro1 in potatoes, Rhg1 / 4 in soybean, and others. We also provide transgenic services, including quantitative trait locus (QTL), the mutant through transgenic complementation, etc.
• Our service lists also include the generation of transgenic plants and genomic subclones, sequence analysis, linkage mapping, gene-specific PCR markers, southern gel blot analysis, genomic libraries, and library screening, and testing for resistance to plant nematodes.

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