Analysis of Active Substances to Kill Nematodes

Microorganisms in the inter-rhizosphere are not only huge in number but also very rich in species, including fungi, bacteria, algae, protozoa, actinomycetes, etc. These organisms and the inter-rhizosphere constitute a special micro-ecosystem. Plant rhizo-microorganisms are a group of microorganisms that colonize the root system and promote plant growth, with a wide variety of species, including fungi, bacteria, and other microorganisms. They can secrete various active substances, including phytohormones, vitamins, amino acids, etc. They can produce antibiotics, extracellular lysozyme, cyanide, iron carriers, etc., thus alleviating and reducing the occurrence of many crop diseases.

Powered by our professional scientists and their years of field experience, Lifeasible provides analysis services of active substances secreted by rhizo-microorganisms to kill nematodes. With our cutting-edge platforms, we can achieve detailed and effective analysis of enzymes, secondary metabolites, and others.

Analysis of Enzymes Secreted by Rhizo-Microorganisms

• The epidermis and eggshell of root-knot nematodes mainly comprise proteins and titin. Antagonistic microorganisms penetrate directly into the root-knot nematode and egg wall, with extracellular enzymes playing a dominant role in the process.
• Lifeasible provides analysis of enzymes secreted by rhizo-microorganisms to kill plant nematodes, including tributylase and protease, etc., that can degrade the nematode body wall and result in nematode death.

Fig.1 Toxins of rhizosphere microorganisms with nematocidal effects.

Analysis of Secondary Metabolites Secreted by Rhizo-Microorganisms

• During the infestation and digestion of root-knot nematodes, in addition to the production of enzymes for ablation, secondary metabolites can be produced that are toxic to root-knot nematode growth and development.
• Some microorganisms can directly infest eggs, but they may also produce secondary metabolites that can inhibit egg development or kill root-knot nematodes. So far, more than 90 toxin substances with nematocidal activity have been identified.
• We provide analysis of secondary metabolites secreted by rhizo-microorganisms to kill plant nematodes by reversed-phase chromatography flow separation technology, such as butyl acetate, 3-isobutylhexahydropyrrole, and others.
• We help our customers to extract nematocidal active substances by the activated carbon extraction method and 732 type cation exchange resin extraction method, and silica gel column chromatography extraction method. We also offer services for determining the purity of nematocidal active substances, including thin-layer chromatography, UV-visible light scanning, and high-performance liquid chromatography.

Lifeasible offers the analysis services of active substances produced by rhizo-microorganisms for your research convenience. We aim to help our clients achieve meaningful results through powerful and consistent approaches. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.