Agricultural Phages Isolation and Purification

Agricultural phages as new antimicrobial agents in the treatment of soil-borne diseases of crops or other products cannot match the advantages of significantly and efficiently improving the survival rate of bacterially infected plants; the idea of using multivalent phages and artificially modified phages to treat bacterially infected diseases has once again become a hot research topic and continues to be refined. The feasibility of using phages to treat soil-borne diseases has been proven and confirmed by extensive field practice, and many phage studies have been reported. Only when phages are isolated and identified can their biological properties and genome structure be analyzed and studied, and detail, and then phages can be better modified and utilized.

With many years of experience in plant physiology, Lifeasible provides professional services for the isolation and purification of agricultural phages, through the isolation and purification of specific phages from samples for subsequent analysis and research to better transform and utilize the phages.

Service content

• Agricultural phages isolation
  Isolation of a specific host phage consists of the following steps.
  • Isolating and culturing out the host bacterium.
  • Infecting the host bacteria with sterile lysate.
  • Proliferation culture.
  • Determining the presence of phage growth by our provided assays.
• Agricultural phage purification
  • When phage spots are present during culture, individual phage spots are selected for culture.
  • Filter, mix the filtrate with the bacterial solution, make a bilayer plate, and continue culturing.
  • Observe the presence of spots and repeat the above steps to obtain purified phage.

Delivery standard

Lifeasible provides a complete experimental report, including the original experimental records, reagents, consumables, experimental conditions, etc. At the same time, our researchers can also provide solutions based on the project itself.

Project cycle

It takes 3-10 days.

Service process

• Service inquiry and technical consultation.
• Sign a contract and fill in the information sheet.
• Sample submission.
• Conduct experiment.
• Delivery of experimental results.

Advantages

• As an expert in soil-borne disease prevention and control, we focus on commercializing agricultural phage resources and have a highly standardized agricultural phage R&D and industrialization platform.
• We are dedicated to the isolation, identification, detection, and culture of agricultural phages, developing valuable agricultural phage resources, and establishing a diverse resource base.
• We have a professional sterile laboratory to ensure an excellent environment for the culture, isolation, and purification of agrophages to avoid unnecessary contamination.
• A professional laboratory team with rich experience in the isolation and purification of agrophages, which can deliver complete experimental results and solutions.