Agricultural phages have great potential for controlling plant bacterial diseases. They are widespread in nature, can self-replicate, target receptors with pathogenic bacteria, are not toxic to eukaryotes, and at the same time, are specific and do not damage beneficial indigenous flora. Given the isolation and technical development of agrophages, Lifeasible can provide biological characterization tests with a full report.
Lifeasible is dedicated to the biological characterization of agrophages based on isolated and purified phage samples. We offer a complete phage testing program that includes optimal infection complex determination, one-step growth curve determination, chloroform sensitivity determination, UV sensitivity determination, UV sensitivity determination, thermal stability determination, pH stability determination, and storage stability determination.
Phage potency is the phage titer, the number of phages with infectious activity per milliliter of sample. Our method for phage potency determination usually uses the double-layer plate method.
The optimal infection multiple is the infection multiple of the offspring phage that gives the highest yield at the end. We take the suspension of R. solanacearum at the logarithmic period and measure the concentration of the suspension, then add the phage according to different infection pluralities, and centrifuge and filter the phage solution for titer determination by bilayer plate method.
The titer was calculated using the double-layer plate method, and the one-step growth curve of the phage was plotted using the logarithm of the phage titer as the vertical coordinate and the incubation time as the horizontal coordinate. The latent period, outbreak period, and lysis amount of the phage were determined based on the one-step growth curve.
Agrophage stock solution and quantitative chloroform were mixed, incubated, and phage potency was determined.
The agrophage stock solution was irradiated vertically under a UV lamp at different times, and the phage potency was measured.
The phage potency was measured by subjecting the agrophage stock solution to different temperatures.
The phage potency was determined by subjecting the agricultural phage stock solution to different pH values.
The phage stock solution is stored at room, low, and high temperatures for a period, and the phage potency is measured periodically.
We provide our customers with complete data reports, experimental protocols, experimental procedures, test conditions, data analysis, etc.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
