Identification and Validation of Small RNAs

Lifeasible is dedicated to developing RNA interference technology in plant protection and provides identification and validation services for small RNAs that can be used for plant protection.

RNAi-based approaches can significantly contribute to integrated pest and pathogen management and sustainable development of agriculture. The first step in using RNA interference to protect plants is to identify RNA interference-associated small RNAs (sRNAs) and determine the silencing targets of sRNAs. The development of second-generation sequencing technologies has accelerated the sequencing of sRNAs and sRNA targets, providing basic expression information and expression differential information. Then, computer-based information analysis also facilitates the identification, clustering, and functional prediction of sRNAs. Furthermore, multi-validation methods of sRNAs help to determine the exact role of sRNAs. Lifeasible makes full use of advanced sequencing technology, comprehensive bioinformatics analysis, and multiple sRNA validation methods to help our customers identify and validate sRNAs for plant protection.

Fig. 1 RNAi-based plant protection strategies (Niu et al., 2021).

Our Services

We provide targeted sRNA identification services.

Table. 1 Identification of sRNAs

Our Approaches

Computer-based sRNA prediction

We can analyze existing genetic information, transcriptomic data, and RNA libraries and predict sRNAs that can be used for plant protection. We have developed an enhanced sRNA scanning method to predict sRNAs efficiently.

Advanced sequencing technology

We use advanced third-generation sequencing and next-generation sequencing technologies to detect nucleic acid sequences for efficient mining of sequence information. We mainly use deep sequencing with Illumina technology for the sequencing of sRNAs.

Comprehensive bioinformatics analysis

Our bioinformaticians can perform a comprehensive analysis of the nucleic acid information. For example, when analyzing RNA data, they use bioinformatics to identify sRNAs present in RNA databases, to predict miRNAs that are not identified, to do hierarchical clustering analysis of differentially expressed miRNAs, to perform multilayered enrichment analysis (GO and KEGG annotation).

Multiple sRNA validation methods

For computer-predicted sRNAs, we will perform sequencing to validate them. For the validation of miRNAs, we mainly use qRT-PCR for validation. We also offer validation using chemically synthesized dsRNAs that then act on the target organism. If there is a demand for cloning validation, we can also provide it.

Lifeasible provides professional sRNA identification and validation services, please contact us to serve you.