Role of Plant Endoplasmic Reticulum in ABA Pathway

Abscisic acid (ABA) was first discovered in the 1960s, it was originally named either dormin or abscisic acid in young cotton fruits and sycamore leaves. Since then, this hormone has been found in a large number of plant species and appears to be a universal occurrence in vascular plants and mosses. ABA plays a key role in seed maturation, acquisition of desiccation tolerance, and dormancy. In addition, ABA regulates many aspects of plant growth and development and plays a central role in response to abiotic stress.

Lifeasible possesses enriched experience in plant science, performing various strategies to help our clients analyze the role of the endoplasmic reticulum in the ABA pathway. Our scientists and expert technicians have been dedicated to customizing your program to meet your requirements.

Role of Plant Endoplasmic Reticulum (ER) in ABA Synthesis

• ABA can circulate throughout the plant as an inactive glucose ester conjugate and then be released into the active form by apoplast and ER β-glucosidase. Since the glucose ester form can be activated by cleavage, this may represent a rapid response mechanism, which always serves as the first defense against volatile environments.
• Lifeasible provides dynamic monitoring of ABA throughout the plant, primarily by tracking the use of two ABA-sensitive promoters fused to a reporter gene consisting of β-glucuronidase or luciferase.

ABA-Mediated ER Stress Response

• ER stress, which in turn activates the unfolded protein response (UPR) to aid in the degradation of refolded or misfolded proteins. And the plant hormone ABA plays a role in transducing stress signals to activate the UPR. bZIP17, as a transmembrane transcription factor acting as an ER stress sensor, plays a role in the regulation of ABA-mediated ER stress response.

Fig.1. Processing for analysis of ABA-mediated ER stress response.

• We provide analysis of overexpression or interference of bZIP17 on ER stress response gene expression, ER stress inducers affecting bZIP17 cellular localization, and help customers analyze the interaction between bZIP17 and ABA response cis-elements.
• We offer biological technologies, containing molecular cloning and construction of plasmids, generation of transgenic plants, RNA extraction, and RT-PCR, western blot analysis, yeast one-hybrid assays, and others.

Lifeasible is pleased to share our cutting-edge technology and extensive expertise in plant science to facilitate our clients' research and project development. We can offer high-quality customized services by adjusting protocols to meet specific requirements. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.