Detection of Signaling in Plant Cells

According to the source of the plant cell signals can be divided into extracellular and intracellular signals. Extracellular signals, also known as first or primary messenger, include extracellular environmental and intercellular signals. Extracellular signals are external environmental factors that affect plant growth and development. Intercellular signal refers to the inter-cellular communication molecules synthesized by the plant body, which can be transported from the synthesis site to other sites and stimulate other cells. Intracellular signals, also known as the second messenger, usually refer to intracellular signaling molecules produced by cells after sensing extracellular signals to regulate cellular metabolism. The intracellular chemical signaling molecules that have been identified so far are small molecules, Ca²⁺, IP3, DAG, cAMP, cGMP, cADPR, NO, and other series of small molecules and their metabolites, which are derived from the degradation of intracellular substrates by effector enzymes.

Lifeasible is committed to plant research, we rely on advanced technology platforms and professional research teams, aiming to work with researchers to promote the progress of plant cell biology research. The plant cell signaling assays we provide are significant in helping researchers uncover new mechanisms of plant cell signaling pathways, understand plant development and improve crop quality.

Applications of plant cell signaling detection

Plant cell signaling detection can help researchers better understand plant cell signaling pathways, which can be further applied to agricultural production, such as improving crop yields, resistance to pests and adversity, and so on. For example, transgenic technology can be utilized to introduce beneficial genes, such as antiviral genes, into crops, which can improve their resistance to pests and diseases, and RNAi and CRISPR-Cas9 technology can be utilized to accurately edit plant genes to improve crop quality and yield effectively. In addition, our plant cell signaling detection can help researchers effectively respond to climate change and environmental stress, providing more reliable technical support for agricultural production. Moreover, the detection of plant cell signaling has an important application value in biomedicine.

What do we offer?

• Detection of calcium signaling in plant cells. Many physiological diseases in horticultural plants are caused by Ca²⁺ distribution imbalance and metabolic disorders, such as pear brown spot and tomato umbilical rot, with impaired fruit quality and reduced economic benefits. In the process of apple production, calcium spraying on young fruits, postharvest calcium dipping, and other treatments can enhance the hardness of apple fruits, delay the softening of fruit senescence, prolong the storage time, and improve the appearance of fruit quality. Ca²⁺ fluorescence imaging is the most intuitive and effective way to study cellular Ca²⁺. For fruit trees that are not easy to establish stable transgenic plants, such as apples and pears, using small-molecule Ca²⁺ fluorescent probes loaded into the cells is very beneficial to study the distribution and changes of Ca²⁺ in the cells.
• Detection of NO signal in plant cells. NO can move rapidly in the cell and can be purged quickly, these characteristics make it an ideal signaling molecule, not only can be used as a molecular switch to regulate a series of intracellular life activities accurately, but also can interact with other signaling molecules to activate and amplify the signaling waterfalls in the cell. In addition to the conventional chemiluminescence and spectrophotometry, electrochemistry, electron paramagnetic resonance spectroscopy, and fluorescence can be utilized to determine the NO content in plant cells. In addition, mass spectrometry and photoacoustic spectrometry are also commonly used for NO detection. Mass spectrometry is used as a rapid, effective, and non-invasive method for the determination of NO released from various organs and tissues or from the whole plant, and the method can recognize nitrogen isotopes. It can measure NO, O₂, and other gases simultaneously from the same sample. Photoacoustic spectrometry is also a non-invasive method for the detection of NO, which is an absorption spectroscopy technique based on the photoacoustic effect.

Our service advantages

Detecting signaling pathways in plant cells can help researchers reveal the role that cell signaling plays in plant growth and development and in response to environmental stresses. Lifeasible provides plant cell signaling detection services currently being used effectively to help researchers conduct studies of Ca²⁺ signaling and NO signaling in plant cells. If you are interested, please feel free to contact us.