Ar.A4 Electroporation Competent Cell

ACC-122

Ar.A4 Electroporation Competent Cell

The genotype of Ar.A4 Electroporation Competent Cell is Agrobacterium rhizogenes(kan R) Ar A4 Ri(agropine type). Agrobacterium rhizogenes is a Gram-negative soil bacterium that can infect most dicotyledons, a few monocotyledons and some gymnosperms. The Ar. A4 Agrobacterium rhizogenes strain contains agrobacterium-type Ri plasmid with a broad host range (corn, tobacco, carrot, licorice, etc.) and kanamycin resistance. This competent cell is particularly suitable for the transformation of large plasmids.

Ar.A4 Electroporation Competent Cell is suitable for transgenic operations of corn, tobacco, carrot, licorice and other plants.

1. Volume of DNA from ligation mix should not exceed 1/10 of the cell mixture; DNA for transformation should be purified and free of organic substances such as ethanol.
2. Do not pipette or vortex cells.
3. Plating volume can be adjusted accordingly.
4. Please avoid excessive use of rifampicin. Maximum concentration of rifampicin in selection is 25 μg/mL.

Store at - 80 °C for 12 months.

Ar.A4 Electroporation: 50 μL/tube * 10 tube/50 tube.

Transformation efficiency of Ar.A4 Electroporation Competent Cell using pK7WGF2 plasmid (spectinomycin resistance) with 50 μg/mL spectinomycin is >10^5 cfu/μg DNA. Transformation efficiency is reduced to half when the plate contains 50 μg/mL spectinomycin and 20 μg/mL rif.