GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags. This antibody is directly conjugated to HRP.
Format
Liquid
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Quantity
40 µg
Reactivity
GST-tagged proteins
Confirmed reactivity
Native and denatured forms of purified GST or GST fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Expected/apparent MW
depends upon fusion partner
Recommended dilution
1: 1: 1000 (ELISA), 1: 2000 (WB)
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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