Protocols

Protocols

Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis

Cellulose acetate membrane electrophoresis is an electrophoresis method that uses cellulose acetate membrane as a support. It is acetate of cellulose, produced by acetylation of the hydroxyl groups of

Agarose Gel Electrophoresis Detection of Plasmids

Agarose gel electrophoresis is an electrophoresis method that uses agar or agarose as a supporting medium. For samples with relatively large molecular weights, such as macromolecular nucleic acids, vi

Transformation of Tomato

Tomato is a plant of the Solanaceae family and is an annual or perennial herbaceous plant. Tomato is one of the most cultivated vegetable crops in the world. Because it has the advantages of self-poll

Transformation of Cucumber

Cucumber originates from the tropical rainforest areas at the southern foothills of the Himalayas. The botanical classification is Cucurbitaceae. Improving existing cucumber varieties through bioengin

Transformation of Arabidopsis thaliana

Principle Arabidopsis thaliana is a cruciferous plant, a biennial herb, 7-40 cm tall. It is widely used in research on plant genetics, developmental biology and molecular biology, and has become a typ

Pollen Tube Pathway-Mediated Cotton Transformation

In recent years, with the continuous improvement of transformation technology, the application of pollen tube pathway in agricultural molecular breeding has become more and more widespread. More than

Transformation of Immature Wheat Embryos Using Gene Gun Method

Principle Gene gun method is also called microprojectile bombardment, parti-cle bombardment and biolistics. The gunpowder-detonated gene gun was first developed by Sanford et al. (1987) of Conell Univ

Agrobacterium tumefaciens-mediated Tobacco Leaf Disk Transformation

Principle Transformation of plant cells by Agrobacterium involves a series of complex reactions, including the attachment of Agrobacterium to plant cells, the release of signal molecules from plant ce

Construction of Tomato PIP Subcellular Localization Vector

Expression of some eukaryotic proteins in prokaryotic host cells is efficient and cost-effective. However, many eukaryotic proteins synthesized in bacteria have low or inactive protein activity due to

Construction of Prokaryotic Expression Vector of Cucumber LOX2 Gene

The method of inserting the cloned foreign gene into a suitable vector and then introducing it into E. coli to express a large number of proteins is generally called prokaryotic expression. Prokaryoti

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