Protocols

Protocols

Tobacco Micropropagation Technology

Tobacco (Nicotiana tabacum) belongs to the Solanaceae plant, is an important industrial raw material, and is also an important one of the four typical experimental plants (tobacco, petunia, carrot, an

Apple Endosperm Culture Technology

Apple is the main tree species cultivated in deciduous fruit trees, and it is also one of the tree species with the widest cultivated area and high yield in the world. Endosperm of angiosperm is one o

Wheat Embryo Culture Technology

Wheat is one of the important food crops, and embryo culture of wheat can not only be used for plant regeneration, but also can break seed dormancy through immature embryo culture to induce dormant se

Chrysanthemum Flower Organ Culture Technology

Chrysanthemum is one of the four largest cut flowers in the world. The high regenerative capacity of floral organ cells makes it a good material for studying cell morphogenesis and also for the study

Tomato Isolated Root Culture Technology

Root culture is mostly used to study root physiological metabolism, organ differentiation and morphogenesis. Root cell cultures can also be subjected to mutagenesis to screen out mutants for plant bre

Media Preparation and Sterilization

Using mother liquor to prepare medium can not only improve work efficiency, but also reduce weighing errors of substances with very small amounts. The medium contains a lot of organic matter, especial

Preparation of Medium Mother Liquor

Before preparing the culture medium according to the culture medium formula, for the convenience of use and accurate dosage, the the macroelements, trace elements, iron salts, organic matter, and horm

Artificial Seed Preparation

Artificial seed is a capsule structure which consist of an embryoid produced by tissue culture techniques and protective substances that can provide enough nutriments and keep the embryoid from spoili

Suspension Culture

Suspension culture is a tissue culture system for culturing single cells and small cell clusters in a constantly agitated or shaken liquid medium, which is used to culture non-adherent dependent cells

Chemical Treatment for Detoxification

Viral diseases have been an important constraint to the sustainability of plant production. The use of virus-free seedlings is one of the effective ways to prevent and control plant virus diseases. De

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