Protocols

Protocols

Construction of Soybean NAC Cloning Vector

Cloning vectors are used to clone and amplify target gene fragments in host cells. This type of vector contains a replication signal sequence, which can replicate in host cells to obtain high-copy DNA

Chromosome Walking Technology in Rosa chinensis

Since the Arabidopsis genome has been sequenced, when studying the promoter of a certain gene, primers can be designed based on the known gene sequence and the promoter sequence of the gene can be dir

5'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s

3'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s

Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a widely used molecular biology technology used to amplify specific DNA fragments in vitro. It can be regarded as special DNA replication outside organisms. Using PC

Extraction of Soluble Protein from Arabidopsis thaliana Leaves

Soluble proteins refer to proteins that can be dissolved in water or other solvents in the form of small molecules. Most of the soluble proteins in plants are enzymes involved in various metabolisms a

Extraction of Total Protein from Tomato Fruit

Protein is the executor of physiological functions and the direct embodiment of life phenomena. The study of protein structure and function will directly elucidate the change mechanism of life under p

Extraction and Purification of Tobacco Leaf RNA

RNA is an intermediate product of gene expression and also the genetic material of RNA viruses. The manipulation of RNA plays an important role in molecular biology. Highly pure and complete RNA is ne

Extraction of Total Protein from Wheat Leaves

Protein is the material basis of life, the basic organic matter that constitutes cells, and the main carrier of life activities. Without protein there is no life. Therefore, it is a substance closely

Extraction and Purification of Rice Genomic DNA

DNA is the carrier of genetic information and the material basis of gene expression. Whether it is to study the structure and function of DNA or to conduct genetic engineering research, the DNA needs

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