Y1H Technology Services

Yeast monohybrid (Y1H) is a technique developed from yeast two-hybrid, mainly used to study the interactions between proteins and DNA elements. The transcriptional activator of many eukaryotes consists of a physically and functionally independent DNA binding region (BD) and a transcriptional activation region (AD). Thus fusion expression vectors for various genes with AD can be constructed. When expressed as a fusion protein in yeast, the protein with a specific binding region to the target element can then be screened based on the expression of the reported gene.

Lifeasible offers Y1H technology service for our customers to study the interactions between biological macromolecules. This service includes a complete experimental workflow covering library construction, one-hybrid screening, and one-to-one validation services.

Y1H library construction and screening service

We use pGADT7 and pGADT7-Rec2 systems for screening. Customers can use pre-made commercial libraries or our custom-built yeast hybrid libraries. We have many years of experience in library construction and screening and have completed thousands of libraries of various types, which can effectively ensure the coverage of libraries and the detection of target genes for library screening.

• Library construction process

• Monohybrid screening process

One-to-one validation service

• Promoter background screening. Binding seq gene promoter background screening.
• One-to-one validation. Set up experimental group (pHis-A+pGADT7-B), positive and negative control.

In addition, we provide further validation solutions for Y1H results, including EMSA (probe synthesis and labeling and EMSA experiments) and dual fluorophore enzyme assay (vector construction and dual fluorophore enzyme assay) to further verify the interaction between gene promoter and protein.

Applications

• Determination of known DNA-protein interactions.
• Isolation of novel genes bound to target cis-regulatory elements or other short DNA binding site proteins.
• Localize DNA-binding structural domains of DNA-binding proteins that have been shown to interact and accurately locate nucleotide sequences bound to DNA.

• One-stop assays are convenient and fast, allowing you to reach reliable conclusions as soon as possible.
• Years of experience in library construction and 4 optimized total RNA extraction protocols to ensure the purity and integrity of total RNA and the diversity of samples.
• Highly efficient library construction techniques can guarantee a positive library rate of 98% or more. The three read frame vectors can be linked to one cDNA sample to achieve zero difference in the quality control index of the three libraries, reducing the impact of library differences on the screening results.

You only need to provide information on the decoy plasmids used for screening, and Lifeasible will complete the entire screening process. We will also provide a detailed and complete experimental report, clear pictures of the experiments, sequencing of the screening results, and plasmids at the end. Please feel free to contact our staff to place your order.