Phyllostachys edulis is an evergreen, renewable forest biomass resource widely used for manufacturing wood, artwork, and papermaking, and biological and pharmacological research, with good economic, food, ornamental, ecological, and medicinal values. Currently, available gene editing systems in bamboo are mainly limited by the dependence on the regenerative capacity of bamboo, which is very time-consuming. A simple and feasible method for rapid gene expression and gene editing is urgently needed for Phyllostachys edulis research due to the unpredictable and very long flowering cycle of Phyllostachys edulis, which usually lasts for several decades of nutrient growth before flowering, which makes it difficult to obtain suitable explant material.
Lifeasible has established a set of methods specifically for the genetic transformation of Phyllostachys edulis based on the challenges faced in Phyllostachys edulis genetic transformation. We have combined Agrobacterium-mediated transient expression methods with the development of a gene editing system that can be effectively applied to Phyllostachys edulis, for example, through our CRISPR/Cas9 technology, which can be used to generate mutants with increased levels of target compounds.
Using Phyllostachys eduli immature embryos as explants, we obtained the medium with the best performance in terms of healing tissue induction rate and adventitious shoot differentiation rate by treating with different combinations of hormone concentrations, and we significantly improved Phyllostachys eduli transformation rate by optimizing the conditions for genetic transformation such as antibiotic screening concentration, the concentration of bacterial infiltration, and the time and manner of infiltration, and so on. eduli transformation rate. In addition, we have cloned several Phyllostachys eduli endogenous promoters, which can be better used for Phyllostachys eduli genome editing. Based on our advanced technology platform and professional plant genetic transformation research team, we have established a higher Phyllostachys eduli in vitro regeneration system, made breakthroughs in Phyllostachys eduli genetic transformation technology, and successfully applied CRISPR/Cas9 technology to Phyllostachys eduli and realized precision editing, which will greatly promote the research of Phyllostachys eduli gene function and transgenic breeding research process.
Bamboo is an important forest resource with very important ecological, economic, and cultural values. Due to the special flowering biology of bamboo plants, it is difficult to breed by sexual hybridization and mainly relies on natural variation to select new varieties. Although the sequencing of Phyllostachys eduli genome was completed a long time ago, and many candidate genes related to economically important traits have been mined by various histological means, the lack of a stable genetic transformation system has limited the genetic transformation in the Phyllostachys eduli somatic validation study. In addition, since Phyllostachys eduli is a representative bamboo species of dispersed bamboo, it is different from clumped bamboo in terms of reproduction and growth habit, and its in vitro regeneration system is more difficult than that of clumped bamboo. Phyllostachys eduli mature embryos can be used to induce healing tissues and differentiate adventitious shoots. However, there are still problems such as difficulty in sterilizing the explants and low regeneration frequency.
Based on the difficulties faced in the genetic transformation of Phyllostachys eduli, we have established an efficient Phyllostachys eduli genetic transformation and gene editing system to characterize the functions of genes regulating economically important traits in Phyllostachys eduli and to obtain new varieties with improved target traits using transgenic means. Therefore, establishing an efficient Phyllostachys eduli genetic transformation and gene editing system is of great significance in analyzing the molecular mechanisms regulating economically essential traits in Phyllostachys eduli and advancing the molecular breeding process of Phyllostachys eduli.
Lifeasible has a complete, large-scale, and standardized technical platform to provide professional and convenient technical services and support for Phyllostachys eduli researchers. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
