Jatropha curcas Transformation

Jatropha curcas is a small tree or shrub-like plant, native to tropical America now, distributed in tropical and subtropical regions worldwide. Jatropha curcas has some medicinal value, in addition to its seeds are the raw materials for the manufacture of soaps, lubricating oils, etc., and its oil meal can be used as pesticides and fertilizers. It can also be used to extract biodiesel. The root system of Jatropha curcas is robust and developed, with strong soil-fixing ability, which makes it useful for the rehabilitation of degraded early slopes and the improvement of ecological and economic functions. Jatropha curcas has a robust and developed root system with strong soil-fixing ability, which is useful for vegetation restoration and ecological and economic functions of degraded early slopes, and several unique properties of Jatropha curcas make it an ideal plant to be used for biodiesel production.

Lifeasible is characterized by a highly efficient and abundant plant genetic transformation system. After years of plant genetic transformation experiments, we have screened the best transformation conditions for Jatropha curcas transformation, and our many years of transformation experience can significantly guarantee the success rate of the experiments. We are currently able to provide various plant genetic transformation services, and we also have gene function research, transgenic detection, and other one-stop transgenic technology services; welcome to consult.

What do we offer?

Jatropha curcas is an auspicious energy plant because of the high oil content in its seeds, which can be developed into "biodiesel", but its distribution area is narrow, its seed yield is low and the quality of its seed oil needs to be further improved. To expand its cultivation area, new high-yielding and high-quality varieties have been bred, and genetic transformation technology can be utilized to achieve these objectives. The transformation of Jatropha curcas involves several key steps.

• Isolation and culture of target cells. Explants, such as leaf or stem tissues, are collected from the plant and sterilized to eliminate contaminants. These explants are then cultured on a suitable growth medium supplemented with plant hormones to induce callus formation.
• Genetic modification. The introduction of foreign genes into Jatropha curcas can be achieved using several techniques. Agrobacterium-mediated transformation is commonly employed, where a modified strain of the soil bacterium Agrobacterium tumefaciens is used to transfer the desired genes into the plant cells. Particle bombardment, also known as biolistics, is another method where DNA-coated particles are shot into the plant cells using a gene gun.
• Selection and regeneration of transformed cells. After gene transfer, selective agents such as antibiotics or herbicides are added to the growth medium to eliminate non-transformed cells. The surviving transformed cells are then induced to differentiate into shoots or somatic embryos under specific culture conditions.
• Rooting and acclimatization. The shoots or somatic embryos obtained from the previous step are transferred to a rooting medium to develop roots. Once the plants have well-developed roots, they are acclimatized to ex vitro conditions by gradually exposing them to the external environment.

Genetic transformation instructions

Customers can directly provide the constructed genetic transformation vector or entrust our company to carry out gene cloning, expression, knockout, and other types of vector construction; we will complete the entire experimental process from the construction of the vector to obtaining transgenic seedlings for you.

Our experimental results

• Jatropha curcas genetic transformation progress description.
• Sequencing report and pictures of vector construction.
• Pictures of explant invasion, induced healing, embryonic healing, tissue differentiation, and plant regeneration.
• Gel electrophoresis diagram for PCR detection of target genes in T0 generation regenerated plants.

Our service process

• Sign a service contract.
• The customer transfers the target gene and makes payment.
• We carry out the transgenic experiment.
• We deliver the experimental results to the customer, and the customer accepts the results.

Why choose us?

Lifeasible is committed to providing reliable technical support for plant genetic transformation. With our advanced plant transformation platforms and experienced team of experts, we can help a wide range of researchers advance the progress of plant transformation research. Let's embrace the era of life sciences with you on the path of scientific research. If you are interested in us, please feel free to contact us.