Luffa Aegyptiaca Miller Transformation

Luffa aegyptiaca miller, also known as loofah, is an annual plant of the Cucurbitaceae family. Currently, loofah is widely cultivated mainly in temperate and tropical regions. Loofah contains a wide variety of nutrients such as protein, fat, carbohydrates, minerals, vitamin B1, vitamin C, saponins, plant mucilage, xylose gum, loofah bitterness, and citrulline. Among them, it is rich in B vitamins that prevent skin aging and vitamin C that whitens skin, which protects the skin and eliminates plaques. The net-like fibers inside the loofah when it is ripe are called loofah, which can be used as a scrubbing sponge instead of a sponge for stoves. Molecular plant engineering methods have more obvious advantages than traditional plant breeding methods. It allows targeted and targeted alteration of plant growth. Genetic transformation of lucerne can more rapidly and comprehensively improve yield, nutritional value, drought resistance, and disease resistance.

What We Offer

Lifeasible has been committed to providing a one-stop service covering all aspects of experimental design, vector construction, plasmid transformation, positive plant screening, and transgenic loofah identification. We mainly provide Agrobacterium-mediated genetic transformation services to help you customize and improve loofah traits. In Lifeasible's loofah genetic transformation service, experts infest loofah cotyledons with Agrobacterium carrying the target gene vector and inserting T-DNA into the genome. After resistance screening according to customers' needs, independent resistant healing tissues are further differentiated and regenerated into transgenic loofah seedlings. In addition, you can select the type of vector construction service you need from the following links.

Service Flow

1. Sample submission requirements

Customers can provide constructed loofah transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable).

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

We have a standardized process for loofah genetic transformation services, along with a team of experts who have been working on plant genetics for many years to ensure professional, efficient, and reliable breeding results. The experts are mainly responsible for providing professional technical advice and experimental guidance assisting customers in project optimization. Our staff will keep you informed when the experiment reaches a critical point in case you miss it. Phase data and results will also be reported to you regularly so that you can always keep track of the progress of the experiment. In addition, we also provide a complete after-sales service for the positive seedlings and are responsible for helping and guiding the transplanting of the seedlings to ensure their survival.

3. Luffa aegyptiaca miller transformation results in delivery

• First you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate).
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram).
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• The advantage of our versatile transformation platform is that it is efficient, stable, and professional, based on the Agrobacterium transformation method, and supports different types of resistance screening.
• We offer different types of Agrobacterium strains and custom vectors, as well as a variety of mutation selection markers that are sure to fit your project needs.
• When you don't have time to manage your breeding work or the season is not right, our unrestricted loofah breeding service will be the best choice.
• Our standardized experimental procedures efficiently drive your research process, ensuring high success rates and short cycle times.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Luffa aegyptiaca miller transformation. We can provide customized solutions to help you study a wide range of loofah varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.