As an industrial leader in genetic modification and engineering of plants, Lifeasible has acquired extensive experience and expert knowledge in RNA interference (RNAi) - the most common means of obtaining sequence-specific gene knockdown.
RNAi introduces double-stranded RNA (dsRNA) into target cells, resulting in rapid and precise impairment of endogenous gene functions. The quickness and general affordability of RNAi have advanced the frontier in plant engineering and crop improvement, leading to the discovery of function-enhanced novel crops, such as nicotine-free tobacco, non-allergenic peanuts, decaffeinated coffee, and nutrient fortified maize.
The RNAi pathway is initiated by the enzyme Dicer, which cleaves long dsRNA into 21-25 nt small interfering RNAs (siRNAs) in target plants. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), of which the guide strand is incorporated into the RNA-induced silencing complex(RISC), followed by argonaute protein-induced target mRNA degradation or translation inhibition at the post-transcriptional level, or block of gene transcription through RNA-directed DNA methylation (RdDM). The RNAi process also can be triggered by microRNA (miRNA) derived from shorter stem-loop RNA within cells through similar mechanisms.
Lifeasible has developed a DNA-vector-based siRNA platform for facile and reliable gene silencing and phenotype modification. As we are committed to quality service and scientific excellence, a client-centric, collaborative approach is guaranteed to ensure that each project is given the attention it needs. Our featured one-stop services intend to accommodate the diverse needs of clients all over the world, including:
For more information or to discuss in detail, please contact us.
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Unique one-stop service
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Various model plant transformation systems
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Optional follow-up analysis services
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Detailed and reliable project reports
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024