Capsicum annuum, also known as chili pepper, is a diploid self-pollinating annual herb belonging to the genus Capsicum in the family Solanaceae. Peppers are native to the Americas and one of the world's oldest vegetable crops. The development of gene sequencing technology and the publication of pepper genome sequences have promoted the application of molecular breeding technology in pepper. Using conventional breeding methods to select highly resistant and high-quality varieties is very difficult and time-consuming, with the result that the genetic diversity of the crop is reduced and beneficial genes are lost. The use of genetic engineering techniques is one of the important methods to improve the yield and quality of pepper.
Lifeasible has been committed to providingproviding one-stop service covering all aspects of experimental design, vector construction, plasmid transformation, positive graft screening, and identification of transgenic peppers. In Lifeasible's pepper gene transformation service, experts use pepper cotyledons as transgenic recipient material, which first needs to be infested with Agrobacterium tumefaciens carrying the target gene vector. T-DNA is then inserted into the genome and screened for resistance in line with the client's needs to obtain independent resistant healing tissues, which are further differentiated and regenerated into transgenic strains. Our various pepper transgenic vector construction services are listed below.
Customers can provide constructed chili transformation vectors directly, and Lifeasible also offers vector construction services.
Concentration | Volume | Other requirements |
80-100 ng/μL | ≥10 μL | no degradation & no contamination |
Bacterial broth | Bacterial plate |
Bacterial broth up to 1-year-old, preserved in glycerol | Bacterial plate activated within one week |
*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.
*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.
*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.
We will keep our clients informed at key points of the genetic modification experiment. You can also contact the staff any time for continuous follow-up of the experimental process. The success of the experiments will be strictly based on the positive identification of the target genes from the plants. All experiments will be performed by our experienced experts under standardized experimental procedures, following strict QC standards.
Lifeasible's experts have comprehensive knowledge and years of experience solving technical problems and challenges in capsicum annuum transformation. We can provide customized solutions to help you study a wide range of pepper varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024