In Vivo Directed Evolution Technology

The core idea of synthetic biology is to design and construct new biological components, networks, and systems based on systems biology, drawing on engineering ideas and modern biological technology methods and eventually artificially reconstructing novel living organisms. As one of the leading technologies in synthetic biology, in vivo directed evolution has significant application prospects in agriculture.

Lifeasible provides in vivo directed evolution strategy that can simplify the experimental operation of directed evolution and reduce human interference, which accelerates the screening of important functional genes.

We offer two new techniques for in vivo directed evolution

Directed evolution refers to the creation of mutations under laboratory conditions and applying screening pressure to a mutant library to screen for mutants with desired phenotypes. We offer the following two new in vivo directed evolution techniques that enable directed evolution and functional screening of genes in plants, which are important for screening important agronomic traits and functional studies of genes in crops.

• PACE technology

A phage-assisted continuous evolution method allows proteins to evolve for 60 rounds every 24 hours by combining the life cycle of biomolecules evolving in the laboratory with that of a phage. This technique is 100 times more efficient than traditional laboratory evolution methods, and the experimental process requires no human intervention, which can greatly save the labor cost of researchers.

• RAGE technology

A class of RNA interference-based genome evolution methods developed in yeast that helps identify 3 knockout targets through 3 rounds of RNAi screening. This technique significantly improves cell acid tolerance through combinatorial knockdown and accelerates the screening of important genes.

Workflow of our directed evolution technique

• Establish the initial protein and the corresponding gene, and introduce mutations in the protein's corresponding gene to achieve a small global coverage of the sequence.
• Transfer the mutated gene to the bacterium so that the different mutant genes segregate and the corresponding mutant protein is expressed in the bacterium.
• The protein mutants with better target performance are screened, and their gene and protein sequences are determined by sequencing means.
• The optimal mutant is used as the parent, and the next "mutation-screening" cycle is followed until the desired protein performance is achieved.

Our service advantages

Lifeasible provides directed evolution as an important technique for generating proteins and nucleic acids with novel functions, facilitating the study of fundamental biological questions, and providing great convenience for the evolution of microorganisms and the evolution of specific proteins or metabolic pathways. At present, our directed evolution techniques have been widely applied to various fields of biotechnology and have greatly accelerated the progress of biotechnology. In vivo directed evolution techniques enable the accumulation and amplification of favorable mutations, saving time and effort and greatly accelerating the evolutionary process. If you are interested in us, please feel free to contact us.