Cucumis Melo Transformation

Cucumis melo, also known as melon, is an annual herb in the Cucurbitaceae family. Melon originated in Africa and has been cultivated for at least 2000 years. Melons can be divided into diploids and polyploids, and the current cultivars are mainly diploid melons. Melon fruits are very sweet and rich in sugars, starch, small amounts of protein, minerals, and vitamins. The whole melon herb can be used as medicine with anti-inflammatory, dehumidifying, and jaundice reducing properties. Currently, melon is one of the most important food crops, and genetically modified biotechnology is being used to improve its yield, fruit storability, and various resistance.

What We Offer

Lifeasible has a professional technical teamthatengaged in plant genetic transformation for many years, committing to providing you with professional melon genetic transformation services. Our plant genetic transformation platform has an efficient experimental process, professional technical service support, and mainstream molecular biology experimental equipment. We use Agrobacterium-mediated transformation to target melon shape modification. Firstly, our experts take melon cotyledons as transgenic recipient material and infest the recipient material with Agrobacterium carrying the target gene vector. Subsequently, after resistance screening, we can obtain independent resistant healing tissues. Finally, the healing tissues will differentiate and regenerate into the target transgenic strain. Our various melon genetic modification services are listed below.

Service Flow

1. Sample submission requirements

Customers can provide constructed melon transformation vectors directly, and Lifeasible also offers vector construction services.

• Customer-supplied plasmid requirements

• Customer-supplied Agrobacterium requirements (Both Agrobacterium broth and plates are acceptable.)

• Mature seeds within one year ≥ 100

*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.

*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.

*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.

2. Experimental process that can be followed at any time

Our entire experimental process is standardized and scalable, allowing for a wide range of customized melon genetic transformation services. Our staff will keep our clients informed at key points in the process and report to you regularly on the current phase of data. All experimental components will follow strict QC standards and be operated by experienced plant geneticists.

3.Cucumis melo transformation results delivery

• First of all, you will receive the T0 positive seedlings that meet your customized needs.
• Detailed DNA sequence identification report (test number, positive vaccine number, total number of positives, positivity rate)
• Information about test primers, vector sequences, variant genes, etc.
• Experimental data and pictures (exosome infestation, induction of healing, embryonic healing, tissue differentiation, plant regeneration, PCR gel electrophoresis diagram)
• Complete, detailed, and comprehensive experimental data and reports.

We Do Better

• Our multifunctional platform for melon genetic transformation is equipped with efficient experimental procedures, professional pre, and post-sales technical support, and complete molecular biology experimental equipment.
• You will have a comfortable and relaxing experience with our laboratory staff, who can customize one-on-one solutions according to your needs.
• Our team of experts is all experienced in the field of plant genetic transformation to ensure a reliable and professional service for you.
• Our melon genetic transformation service is based on the mainstream Agrobacterium-mediated method, which is fast, efficient and stable, and supports screening for resistance of Chaumycin, G418, Basta, etc.

Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in cucumis melo transformation. We can provide customized solutions to help you study a wide range of melon varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.