Apium graveolens L., commonly known as celery, is a perennial herb of the genus Celery in the Umbelliferae family. Celery is a vegetable widely grown throughout the world and is rich in dietary fiber, many vitamins, many mineral elements, and very beneficial ingredients for the human body. In addition, celery is rich in organic acids and apigenin, from which apigenin A and apigenin B can be isolated. Modern research has shown that apigenin has many medicinal uses, including lowering blood pressure, lowering blood lipids, preventing cancer, preventing atherosclerosis, and inhibiting bacteria. With the development of molecular biology, genetic engineering can compensate to a certain extent for the shortcomings of traditional breeding, but it also has the advantages of simple operation, high transformation rate, and short cycle time. The application of molecular biology techniques provides new tools for studying genetic functions and genetic improvement of celery.
Lifeasible is committed to providing you with a genetic transformation system for celery based on Agrobacterium transformation method with high stability, high transformation efficiency, high success rate, and short cycle time. Our one-stop custom transformation service is optimized by experts for years, including experimental design, vector construction, plasmid transformation, positive plant screening, and PCR identification. Agrobacterium-mediated transformation is known to be the best method for introducing genes into the celery genome. With rich experience in plant transformation, we have developed several different types of Agrobacterium-mediated gene transformation methods, such as GV3101, AGL-1, EHA101, EHA105, C58C1, etc. Moreover, for vector customization, we provide various vector structures, as shown below, for your choice.
Customers can provide constructed celery transformation vectors directly, and Lifeasible also offers vector construction services.
| Concentration | Volume | Other requirements |
| 80-100 ng/μL | ≥10 μL | no degradation & no contamination |
| Bacterial broth | Bacterial plate |
| Bacterial broth up to 1-year-old, preserved in glycerol | Bacterial plate activated within one week |
*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.
*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.
*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.
We have a standardized and scalable genetic transformation process for celery that allows us to provide targeted and customized services to our customers. All experimental procedures will follow strict quality control standards and be operated by experts who have been involved in plant genetics research for many years. Our staff will keep our customers informed by email when critical points of the experiments are available in case you miss them. In addition, we will also keep you informed of progress and data on a regular basis so that you are well informed of the experiment.
Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Apium graveolens L. transformation. We can provide customized solutions to help you study a wide range of celery varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
