Lilium has not only good ornamental value but also edible and medicinal functions. Due to the lack of a stable and efficient genetic transformation technology system, the research on the gene function of Lilium and germplasm innovation through molecular means has been greatly restricted for a long time. So far, about dozens of multiple methods have been used to establish genetic transformation receptor systems for Lilium, a perennial bulbous plant. With the development of molecular biology, the ability to produce new Lilium varieties with stress resistance, and disease and good quality through transgenic methods is of great significance for Lilium breeding.
Based on our advanced plant transformation platform and experienced research team, Lifeasible provides complete technical support services for Lilium tissue culture and genetic transformation. This includes the establishment of nutrient organs, reproductive organs, and protoplast regeneration systems. As well as Lilium transformation using Agrobacterium, gene gun, electro-excitation perforation and other methods.
We established a genetic transformation system for Lilium using the Agrobacterium rhizogenes-mediated genetic transformation method. Concentrations of screening agents suitable for Lilium transformation, strain selection, determination of the concentration of the bacterial solution, infiltration time, and other transformation conditions were determined to achieve the highest transformation efficiency for Lilium.
Currently, the main methods we applied to Lilium gene transformation are the Agrobacterium-mediated method, gene gun method, and electro-excitation method. Agrobacterium-mediated method is the most widely used in Lilium genetic transformation. While the gene gun method is a new method of genetic transformation emerging in recent years, the research of the gene gun method in Lilium genetic transformation is mainly to study the expression of marker genes in the recipient system. In addition, the electrostimulation method utilizes the pulses generated by high-voltage discharges to create reversible "micropores" in the cell membrane, thus allowing exogenous DNA and other macromolecules to enter the cell through this channel.
Establishment of an efficient regeneration system and genetic transformation of Lilium. We have established an efficient regeneration system for Lilium using its leaf tissue as an explant. It was found that the differentiation ability of Lilium leaf tissues was stronger than that of petiole tissues. We used the Agrobacterium rhizogenes-mediated method to transform Lilium with a target gene and obtained a batch of thaumatin-resistant plants through screening and differentiation induction. Finally, positive plants were obtained using PCR assay. This technical support service we provided provides a research basis for carrying out Agrobacterium-mediated genetic transformation of Lilium on a large scale and improving the quality of Lilium.
In various improvement studies based on Lilium genetic transformation, we not only detect the integration of exogenous genes but also observe the stable, normal, and high-level expression of exogenous genes.
Lifeasible provides state-of-the-art Lilium transformation services and the technical support and solutions we offer play an important role in helping researchers advance the process of Lilium research and application and realize the full potential of Lilium. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
July 13, 2024
