Liriodendron chinense Transformation

Liriodendron chinense is a genus of Magnoliaceae. Distributed in China and northern Vietnam, it has peculiar leaf shape and colorful flowers and is an excellent ornamental tree species for garden cultivation and landscaping. At the same time, its tree body is tall; the wood structure is detailed and uniform, it is an excellent pulp and paper, man-made boards and furniture timber species, and the social demand is excellent. But hybrid Liriodendron chinense growth cycle is long, and the application of conventional means of breeding has more significant difficulties. Therefore, it is necessary to rely on modern biotechnology and combine it with conventional breeding to shorten the breeding cycle, accelerate the breeding process, create high-quality plantation forests, and alleviate the contradiction between timber supply and demand.

Lifeasible is a comprehensive phytotechnology service company; based on our advanced plant transformation platform and experienced research team, we aim to provide complete technical support services for plant genetic transformation. We have conducted Liriodendron chinense genetic transformation research based on the Liriodendron chinense somatic embryogenesis system and applied genetic engineering to Liriodendron chinense-directed breeding research. This technical support service has opened up a new way to genetically improve forest trees.

What do we offer?

Agrobacterium-mediated genetic transformation of Liriodendron chinense. Agrobacterium-mediated genetic transformation is the most successful and widely used method for entering exogenous DNA into plant cells. Agrobacterium-mediated transformation of Liriodendron chinense offers a valuable tool for introducing new traits into the species, such as disease resistance or improved growth characteristics. It allows researchers to study gene function and regulation in Liriodendron chinense and has potential applications in plant breeding and genetic improvement efforts.

Gene gun-mediated genetic transformation of Liriodendron chinense. To address the deficiencies in the prior art, we have also established a gene gun-mediated transformation system for Liriodendron chinense, using embryonic healing tissues and suspension cell lines of Liriodendron chinense as the receptor materials for genetic transformation of the gene gun to set up a highly efficient and stable genetic transformation system and to improve the transformation efficiency of the gene gun. It includes the following parts.

• Take the embryonic healing tissue of hybrid Liriodendron chinense and subject it to hyperosmotic treatment.
• Extraction of plasmid, preparation of gold powder, and preparation of micro bomb.
• Gene gun bombardment.
• Transferred the healing tissue and subjected it to a dark recovery culture.
• Somatic embryo induction culture, light culture after growing somatic embryo, seedling growth, and inoculation in rooting medium for screening culture
• A molecular assay was performed to obtain the Liriodendron chinense transformation system.

The method we established for the gene gun-mediated transformation system of Liriodendron chinense provides the feasibility of the gene gun transformation system of Liriodendron chinense and also provides a reliable experimental basis for the future use of gene gun-mediated transformation of Liriodendron chinense with target genes.

What do customers need to provide?

• Customers can directly give the constructed plant transformation vector, or let our company help you with vector construction.
• Subject to various factors, the experimental cost may be adjusted from time to time; please refer to the reply for the specific experimental cost of direct consultation and contact.
• We can carry out experiments throughout the year and welcome inquiries from researchers.

Our project report

• Vector construction sequencing report and pictures.
• Pictures of explant invasion, induced healing, embryonic healing, tissue differentiation, and plant regeneration.
• Gel electrophoresis graphs of PCR assay of target genes in T0 generation regenerated plants.

Why choose us?

• High success rate. Our advanced plant transformation platform and experienced professional technical team significantly guarantee the success rate of the experiment.
• Multiple plant transformation platforms. We provide gene transformation technology services covering a wide range of plants, which can significantly meet the research needs of researchers.
• Efficient experimental process. Our experimental technology platform allows us to carry out gene transformation projects at any time, and we use a variety of transformation methods which can quickly and efficiently carry out plant gene transformation research.

Our service workflow

Lifeasible leads the industry in plant transformation. We are constantly optimizing our plant transformation technology platform to ensure high transformation success rates. We offer customized plant research services, allowing our clients to make individual requests for project proposals and delivery criteria, and we will provide you with customized solutions to ensure that your experimental needs are met to the fullest extent possible. If you are interested in us, please feel free to contact us.